PUNS: transcriptomic- and genomic-in silico PCR for enhanced primer design
نویسندگان
چکیده
UNLABELLED We developed a CGI/Perl-based web server to perform in silico polymerase chain reaction (PCR) on PCR primer sequences. The PUNS (Primer-UniGene Selectivity) server simulates PCR reactions by running BLASTN analysis on user-entered primer pairs against both the transcriptome and the genome to assess primer specificity. PUNS is particularly suited for the identification of highly selective primers for quantitative microarray validation. AVAILABILITY Both system access and source-code are freely available at http://okeylabimac.med.utoronto.ca/PUNS.
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ورودعنوان ژورنال:
- Bioinformatics
دوره 20 15 شماره
صفحات -
تاریخ انتشار 2004